在虫感染期间招募和维护CX3CR1+CD4+T细胞
Denis G Loredan1,2, Joseph C Devlin1, Kamal M Khanna1,3
1Department of Microbiology, New York University Grossman School of Medicine, New York, NY.
Journal of immunology (Baltimore, Md. : 1950)
|January 5, 2024
概括
在虫感染期间,CX3CR1+CD4+T细胞被激活和组织定位. 转录因子BCL6对于在感染期间维持这些CX3CR1+CD4+T细胞至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
背景情况:
- 清晰的T淋巴细胞子集在炎症期间表达CX3CR1.
- 在2型炎症中CX3CR1+CD4+T细胞的作用,特别是在虫感染期间,仍然在很大程度上未被描述.
研究的目的:
- 在小鼠中,在急性 (Nippostrongylus brasiliensis) 和慢性 (Schistosoma mansoni) 虫感染期间表征CX3CR1+CD4+T细胞.
- 研究这些细胞的表型,组织定位能力,细胞因子生产和生存优势.
- 确定关键的分子调节器,如BCL6,参与它们的维护.
主要方法:
- 利用一个命运映射小鼠模型来追踪CX3CR1+CD4+T细胞.
- 采用单细胞RNA测序来分析细胞多样性并识别不同的种群.
- 进行了条件基因删除 (BCL6),以评估其在T细胞维护中的作用.
主要成果:
- 鉴定出CX3CR1+CD4+ T细胞是具有可变细胞因子生产的激活,组织定位子集.
- 维持CX3CR1和TH2表型在炎症组织中赋予了生存优势.
- 单细胞RNA测序揭示了显著的异质性,并确定了脏中的新型BCL6+TCF-1+PD1+CD4+T细胞群.
- 条件删除BCL6减少了感染期间CX3CR1+CD4+T细胞的数量.
结论:
- CX3CR1+CD4+T细胞是一种异质的,激活的子集,在虫感染期间的2型炎症中很重要.
- 在虫感染期间,BCL6在这些T细胞的维持和持续反应中发挥着关键作用.
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