使用基因组编辑的Corynebacterium glutamicum使用CRISPR-Cpf1系统从软木中生产酸
Dae-Seok Lee1, Eun Jin Cho1, Dinh-Truong Nguyen2
1Bio-energy Research Institute, Chonnam National University, Gwangju, Republic of Korea.
Biotechnology journal
|January 5, 2024
概括
经过工程设计的Corynebacterium glutamicum从软木水解酸中产生了酸. 一种突变菌株达到14.82 g/L,而一种共同表达变换剂达到39.67 g/L,表明可持续生化生产的潜力.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 谷氨酸菌 (Corynebacterium glutamicum) 是一个用于工业生物工艺的多功能微生物.
- 酸是一种有价值的平台化学物质,具有多样化的应用.
- 从可再生资源有效生产酸对于可持续发展至关重要.
研究的目的:
- 设计Corynebacterium glutamicum以从软木水解剂中增强酸生产.
- 调查乳酸脱酶1 (ldhA) 基因淘汰和酸载体共表达的作用.
- 用预处理的软木基板来优化酸生产.
主要方法:
- 使用CRISPR-Cpf1基因组编辑生成了一个ΔldhA突变体和一个共同表达变变体 (Psod:SucE-ΔldhA).
- 在软木 (Pinus densiflora) 的酶性水解中使用HPAC预处理.
- 发酵实验在批量和食批量系统中进行,以评估酸生产.
主要成果:
- 突变型 ΔldhA 在6小时内从4%的软木水解酸中产生了14.82g/L的酸,没有检测到酸或乳酸.
- 同表达变换剂[Psod:SucE-ΔldhA]在6小时内实现了17.70g/L的酸.
- 在食批次系统中,在48小时内产生了39.67g/L的酸,产量为0.77g/g的糖,消耗了100%的葡萄糖和73%的树脂糖.
结论:
- 经过工程改造的Corynebacterium glutamicum菌株可以有效地从软木水解酸中产生酸.
- 开发的代谢工程策略显著提高了酸标位和产量.
- 这项研究突出了利用红纤维素生物质为可持续酸生产的潜力.
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