相关实验视频

Updated: Jul 6, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

TGFB1I1促进细胞增殖和迁移在尿癌

Peir-In Liang¹, Yu-Ching Wei^2,3, Huan-Da Chen^1,4

¹Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

The Kaohsiung journal of medical sciences

|January 5, 2024

概括

转化生长因子β-1-诱导的转录1 (TGFB1I1) 促进尿路细胞癌 (UC) 的攻击性. 过度表达TGFB1I1与晚期瘤相关,并通过诱导上皮层-介质细胞过渡 (EMT) 来促进细胞迁移.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
泌尿器细胞癌的癌症.

背景情况:

尿道癌 (UC),特别是上泌尿道尿道癌 (UTUC),在全球和台湾普遍存在.
确定UC可靠的预后标志物对于患者的治疗结果至关重要.
转化生长因子β-1-诱导的转录1 (TGFB1I1) 涉及到诸如上皮层-介质细胞转换 (EMT) 这样的细胞过程.

研究的目的:

调查TGFB1I1作为尿路癌 (UC) 的预后标记物的作用.
确定TGFB1I1表达和上泌尿道尿癌 (UTUC) 和尿膀尿癌 (UBUC) 的临床病理特征之间的相关性.
为了阐明TGFB1I1在体外对UC细胞行为和EMT的影响.

主要方法:

从UTUC和UBUC患者的in silico和临床病理学数据的分析.
免疫组织化学 (IHC) 染色以评估TGFB1I1的表达.
在体外研究评估TGFB1I1对UC细胞增殖,活力,迁移和EMT标记物的影响.

主要成果:

过度表达TGFB1I1显著与晚期瘤阶段,乳头状结构和UTUC频繁的线粒分裂相关.
在UBUC中,TGFB1I1过度表达与晚期瘤阶段和更高的组织学等级有关.
在体外,TGFB1I1敲击降低了UC细胞的增殖,活力,迁移和EMT标志物.

结论:

TGFB1I1在UTUC和UBUC中过度表达,与侵袭性瘤特征相关.
TGFB1I1通过EMT诱导促进UC细胞的增殖,活力和迁移.
TGFB1I1 作为一种潜在的预后标志物和治疗点,可以促进UC的瘤攻击性.

关键词:

转化生长因子β-1-诱导的转录 1 1 上部泌尿道泌尿瘤 (UROTHELIAL CARCINOMA) 是一种尿道上部的癌症.泌尿膀泌尿细胞癌是什么意思

更多相关视频

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

相关概念视频

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Activation of Integrins

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...