一个高的催化效率和耐化学物质形成脱酶从简单的Bacillus
Rattima Boonkumkrong1, Paweenapon Chunthaboon1, Pobthum Munkajohnpong2
1Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Biotechnology journal
|January 5, 2024
概括
简单杆菌形式脱酶 (BsFDH) 比其他酶具有更高的活性和稳定性. 这种强大的酶非常适合工业生物技术,特别是在辅因子再生和生物催化过程中.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 工业生物技术 工业生物技术
背景情况:
- 依赖NAD+的形式脱酶 (FDH) 在酶合成中对NADH再生至关重要.
- 目前的FDH酶通常具有较低的活性和稳定性,限制了工业应用.
- 需要更强大,更有效的FDH酶用于大规模生物催化.
研究的目的:
- 来自简单菌 (BsFDH) 的FDH的生物化学和催化特性.
- 为了比较BSFDH与Pseudomonas sp.中常用的FDH. 101 (PsFDH). 这是一个很好的例子.
- 评估BSFDH适用于工业应用的适用性,包括辅因子再生和H2O2耐受性.
主要方法:
- BsFDH和PsFDH的生物化学表征,包括动力学分析 (kcat).
- 在不同温度和pH值下评估酶活性.
- 对酸和过氧化 (H2O2) 等抑制剂的酶稳定性和耐受性的评估.
- 在生物催化级联中证明BSFDH用于4-尼托醇排毒.
主要成果:
- 与PsFDH (7.7 ± 1.0 s-1) 相比,BsFDH表现出显著更高的形式氧化活性 (kcat = 15.3 ± 1.9 s-1).
- 在60°C时,BsFDH表现出最佳活性,比PsFDH大6倍.
- BsFDH表现出增强的pH稳定性和对亚酸 (200倍以上的基) 和H2O2.2的优越耐受性.
- 使用BsFDH的生物催化级联由于其H2O2耐受性而显示出优异的转化活性.
结论:
- BsFDH是一种高度活跃和强大的酶,与通常使用的FDH相比具有显著的优势.
- 其增强的稳定性和对恶劣条件的耐受性使其适合于工业生物技术.
- 在辅因子再生和生物催化过程中,BSFDH具有很大的应用潜力,特别是那些涉及H2O2副产品的生物催化过程中.
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