高性能液态染色法用于确定使用弱紫外线染色体的药物的纯度 - - 综述
Elżbieta Sobolewska1,2, Magdalena Biesaga1
1Faculty of Chemistry, University of Warsaw, Warsaw, Poland.
Critical reviews in analytical chemistry
|January 5, 2024
概括
这篇评论比较了高性能液态染色学 (HPLC) 检测器,用于分析具有弱染色体的药物. 它强调了超越紫外线/Vis检测的方法,以确保药物质量并帮助分析开发.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
- 染色体学 染色体学是什么
背景情况:
- 高性能液体染色学 (HPLC) 对于分析复杂的新药至关重要.
- 紫外线/紫外线探测器是常见的,但仅限于具有染色体的化合物.
- 分析具有弱染色体的化合物是一个重大的分析挑战.
研究的目的:
- 用HPLC为具有弱染色体的化合物提供分析方法开发的概述.
- 为了比较各种适合分析具有有限染色体的化合物的HPLC检测技术.
- 协助科学家对候选药物和质量控制进行分析开发.
主要方法:
- 2006年至2023年间发表的基于HPLC的方法的文献综述.
- 在PubMed,Scopus,Semantic Scholar和ScienceDirect数据库中进行的搜索.
- 不同HPLC检测器的分析和比较,包括UV/Vis,FLD,CAD,ELSD,RID,ECD,CLND和MS.
主要成果:
- 确定了超越UV/Vis的各种HPLC检测方法,用于分析具有弱色素的化合物.
- 包括诸如光探测器 (FLD),充电气溶探测器 (CAD),蒸发光散射探测器 (ELSD),折射率探测器 (RID),电化学探测器 (ECD),化学发光探测器 (CLND) 和质谱 (MS) 等探测器.
- 在科学文献中证明了这些探测器的适用性和比较.
结论:
- 有几种HPLC检测技术可用并且有效用于分析具有弱色素的化合物.
- 本综述为在药物分析中选择合适的检测器提供了有价值的见解.
- 这些发现支持改善候选药物的研究和质量保证.
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