HTSplotter:一个端到端的数据处理,分析和可视化工具,用于化学和遗传体外干扰查
Carolina Nunes1,2,3,4, Jasper Anckaert3,5, Fanny De Vloed2,3
1Lab for Computational Biology, Integromics and Gene Regulation (CBIGR), Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
PloS one
|January 5, 2024
概括
HTSplotter自动化数据分析和可视化,用于各种高通量查实验,包括复杂的遗传化学查和实时测试. 该工具提高了处理大型生物数据集的效率和准确性.
科学领域:
- 生物医学研究的研究.
- 计算生物学是一种计算生物学.
- 药物发现 药物发现
背景情况:
- 高通量查 (HTS) 通过自动化,高效的数据生成加速生物医学研究.
- 当前的HTS数据分析工具往往缺乏多功能性,无法支持各种实验设置,如实时测试或组合遗传化学屏幕.
- 对于HTS而言,手动数据处理是繁重,耗时且容易出现错误的.
研究的目的:
- 开发一种自动化,多功能工具,用于分析和可视化来自各种高通量选实验的数据.
- 通过实时测定和组合遗传化学查,解决现有工具的局限性,从而实现实时测定和组合遗传化学查的分析.
- 为研究人员提供一个高效和准确的解决方案,用于端到端的HTS数据处理.
主要方法:
- 开发了HTSplotter,这是一个用于自动分析和可视化HTS数据的Web工具和Python模块.
- 实施了一种算法,用于自动识别实验类型和控制.
- 综合数据规范化 (包括增长率) 和下游分析 (剂量反应,使用Bliss独立性的协同作用,零相互作用强度,最高单剂方法).
主要成果:
- HTSplotter成功地处理了药物,药物组合,遗传干扰物和遗传化学干扰物屏幕的终点和实时测定.
- 该工具自动执行数据规范化和关键下游分析.
- 结果以文本文件形式导出,可视化以PDF格式保存.
结论:
- 对于高通量选数据分析,HTSplotter在多功能性和效率方面提供了显著的改进.
- 该工具能够自动分析遗传化学干扰物屏幕和实时测定随着时间的推移是一个关键优势.
- HTSplotter简化了体外细胞培养查数据的端到端工作流,节省时间和减少错误.
相关概念视频
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:16In vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:46Genetic Screens
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...