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Updated: Jul 31, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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在细胞-矩阵接口处对人类初级和干细胞进行超谱映射

Emiliana De Santis1, Nilofar Faruqui1, Craig T Russell2

  • 1National Physical Laboratory, Hampton Road, Teddington TW11 0LW, U.K.

ACS applied materials & interfaces
|January 5, 2024
PubMed
概括

超光谱成像揭示了矩阵材料化学如何影响干细胞生物化学. 这种方法可以绘制脂质和蛋白质含量的变化,这对于开发先进的生物医学材料至关重要.

关键词:
生物材料是一种生物材料.胚胎干细胞是一种胚胎干细胞.细胞外矩阵是细胞外矩阵.频谱成像技术的使用.同步子辐射显微光谱学组织工程是组织工程.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.