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在PGS/PCL支架上,视网膜质细胞轴突的增长由纤维神经变因子介导

Sanaz Behtaj^1,2,3, Fereshteh Karamali², Samaneh Najafian²

¹School of Engineering and Built Environment, Griffith University, Engineering Drive, Southport QLD 4222, Australia.

Biomedical materials (Bristol, England)

|January 5, 2024

概括

在PGS/PCL支架上状神经变因子 (CNTF) 的共振固定增强了视网膜质细胞 (RGC) 神经质扩展. 这种仿生方法显示了在视神经受伤后改善RGC再生的潜力.

科学领域:

生物材料科学生物材料科学
神经科学是一个神经科学.
组织工程是组织工程.

背景情况:

状神经变因子 (CNTF) 支持神经元的生存和分化,特别是视网膜质细胞 (RGCs).
有效地将CNTF输送到目标组织,如视神经,对于其治疗疗效至关重要.
聚合物支架正在探索受控的生长因子传递,但它们在RGCs CNTF释放中的作用仍未得到充分研究.

研究的目的:

为了研究CNTF对电多 (?? 甘醇脂酸盐) /多 (?? 卡普罗拉克) (PGS/PCL) 支架的共价固定.
评估固定CNTF促进RGC神经元外生和轴突生长的能力.
与自然细胞外基质相比,评估 CNTF 泄露的 PGS/PCL 支架的生物模拟性.

主要方法:

电PGS/PCL生物聚合物支架的制造.
在PGS/PCL支架上对CNTF进行共价固定.
使用在脚手架上培养的RGC爆炸物建立出活体动物视神经模型.
在培养20天后,使用特定的测量技术量化神经元外生.

主要成果:

在CNTF介导的环境中培养的RGC拓展物显示神经元扩展显著增加.
在PGS/PCL支架上的固定CNTF表现出受控释放,模仿自然的细胞外基质.
该PGS/PCL支架促进了CNTF在促进RGC轴突再生中的作用.

结论:

在PGS/PCL支架上对CNTF的共价固定是一种增强RGC神经元延伸的可行策略.
CNTF-eluting支架的生物仿真性质支持RGC轴突的再生.
这种方法有望提高RGC再生疗法对视神经损伤的疗效.

关键词:

生物聚合物是一种生物聚合物.状神经变因子是什么意思梯度释放放出梯度释放的时间.视神经的视觉神经是如何工作的再生医学是一种再生医学.视网膜质细胞 (RGCs) 是一种细胞.

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