相关实验视频

Updated: Jul 6, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

下调circ_0001681 通过负调节miR-56767预测肺癌的发生和恶性发展

Min Li¹, Changmin Mao², Wenjuan Li¹

¹Department of Internal Medicine, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Annals of clinical and laboratory science

|January 5, 2024

概括

降低circ_0001681水平是肺腺癌 (LUAD) 发生和进展的生物标志物. 这一发现为改善LUAD治疗策略提供了潜在的治疗目标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

肺腺癌 (LUAD) 的查和监测对于有效治疗至关重要.
识别新的生物标志物可以改善LUAD治疗策略.

研究的目的:

使用GSE146689数据集,探索circ_0001681作为LUAD的新生物标志物.
评估circ_0001681在LUAD中的功能作用及其作为治疗点的潜力.

主要方法:

定量PCR (qPCR) 用于评估126名LUAD患者,75名良性肺病患者和57名健康个体的circ_0001681水平.
接收器运行特征 (ROC) 和考克斯回归分析用于诊断和预后意义.
细胞计数套件-8 (CCK8) 和Transwell测定用于研究circ_0001681对LUAD细胞增殖和运动性的影响.

主要成果:

与对照组相比,LUAD患者的circ_0001681水平显著降低,显示出诊断潜力.
循环0001681的下调与LUAD严重程度,恶性瘤和不良预后相关.
circ_0001681对miR-567进行负调节,通过其对LUAD细胞增殖和运动的抑制作用进行调节.

结论:

降低circ_0001681功能作为LUAD发展的生物标志物.
circ_0001681有可能被纳入LUAD治疗策略的制定和调整.

关键词:

环绕RNAss 的时间.诊断诊断诊断诊断诊断诊断没有编码的RNAs.非小细胞肺癌的肺癌.预后预后预后

更多相关视频

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.