基于点放电原子排放光谱学合与金丝丰富的小型甲酸测试平台
Yanping Wang1, Yuemei Chen1, Kejun Li1
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Analytica chimica acta
|January 5, 2024
概括
一个新的便携式系统增强了微等离子体原子发射光谱 (AES) 对于敏感的生物测试. 这种方法通过测量离子来间接检测酸 (AA),从而实现潜在的临床检测的低检测极限.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 生物技术是生物技术.
背景情况:
- 微型化基于微等离子体的原子发射光谱 (AES) 为元素分析提供了优势,但缺乏对生物测试的敏感性.
- 开发灵敏的,便携式微等离子体AES生物测试方法仍然是一个挑战.
研究的目的:
- 开发一种简单,灵敏,便携的基于微等离子体的AES生物测试系统.
- 建立一个间接的分析策略来检测 Askorbic 酸 (AA).
主要方法:
- 一个便携式的点排放化学蒸汽生成原子发射光谱 (PD-CVG-AES) 系统与金丝丰富相结合.
- 亚酸通过监测 (Hg2+) 离子度的变化间接检测到.
- 使用一种涉及银 (Ag+) 和锡 (II) 化物 (SnCl2) 的新反应机制,在对AA存在的反应中放大Hg2+信号.
主要成果:
- 该系统实现了亚酸的低检测极限 (LOD) 19 nM.
- 在Hg2+和AA检测方面都表现出高灵敏度.
- 在AA测量中获得了0.7%的相对标准偏差 (RSD).
结论:
- 开发的分析策略显著扩大了基于微等离子体的AES用于生物测试的应用.
- 该方法在传统中医药 (TCM) 中对AA确定具有很高的灵敏度.
- 该系统显示了未来医疗点检测 (POCT) 应用的潜力.
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