对Onobrychis viciifolia的染色体级基因组组装揭示了基因复制数的增加,这是基础上增强的proanthocyanidin生物合成
Junyi He1, Danyang Tian1, Xue Li1
1College of Grassland Science and Technology, China Agricultural University, 100193, Beijing, China.
Communications biology
|January 5, 2024
概括
料作物Sainfoin具有新组装的基因组,揭示了对高西安丁 (PA) 含量负责的基因扩张. 这一发现有助于理解PA生物合成和豆类进化.
科学领域:
- 植物基因组学 植物基因组学
- 料科学是一种料科学.
- 生物化学 生物化学
背景情况:
- 圣草 (Onobrychis viciifolia) 是一种有价值的多年料作物,被称为"圣草".
- 它含有丰富的原始蛋白质和普罗安东基亚尼丁 (PA),也被称为凝结宁.
- PAs具有有益于动物料的药理性质,包括减轻小病.
研究的目的:
- 为了执行一个高质量的,染色体水平的基因组组装的autotetraploid常见的sainfoin.
- 为了调查 sainfoin.in 中高含 proanthocyanidin (PA) 的遗传基础.
- 为了比较与PA生物合成相关的基因拷贝数量和表达与其他Fabales物种.
主要方法:
- 在染色体层面的基因组组合的自动四倍体 sainfoin (2n=4x=28).
- 对参与PA生物合成的基因拷贝数进行比较分析.
- 转录组分析以评估与PA含量相关的基因表达.
主要成果:
- 产生了一种高质量的sainfoin基因组组 (~1950.14 Mb,28个伪染色体,四个单基因类型).
- 与Medicago sativa,Lotus japonicus,Medicago truncatula和Glycine max相比,Sainfoin的PA生物合成基因拷贝数量显著更高.
- 基因扩张被确定为PA基因表达增加和SAINFOIN中PA积累增强的主要驱动因素.
结论:
- 圣福因基因组组合为了解豆类基因组演变提供了宝贵的资源.
- 基因扩张和基因表达的增加是sainfoin高PA含量的关键因素.
- 这项研究增强了我们对料豆类中二次代谢物生物合成的知识.
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