阿斯珀吉勒斯阿瓦莫里MK788209细胞酶:生产,统计优化,豆皮糖化和织应用
Faten A Mostafa1, Hala R Wehaidy1, Samar Sharaf2
1Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, 12622, Egypt.
Microbial cell factories
|January 5, 2024
概括
这项研究优化了使用阿斯伯吉勒斯 (Aspergillus awamori) 来从农业废物中生产细胞酶. 由此产生的酶有效地糖化了豆皮,并改善了织应用的棉织物特性.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么? 酶工程是什么
- 工业微生物学 工业微生物学
背景情况:
- 在酶市场上,对具有成本效益的纤维化酶合成的需求日益增长.
- 需要可持续地利用农业废物用于工业酶生产.
研究的目的:
- 为了从农业废物中生产细胞质,使用阿斯伯吉勒斯 (Aspergillus awamori).
- 研究该酶在糖化和织工业中的有效性.
主要方法:
- 固态发酵 (SSF) 使用Molokhia茎通过阿斯伯吉路斯 awamori MK788209.9.的细胞酶生产.
- 使用Plackett-Burman (PB) 和中央复合设计 (CCD) 的统计优化,以提高酶产量.
- 在豆皮的糖化和棉织物的处理中应用优化的酶.
主要成果:
- 通过统计优化实现了细胞酶产量的4.86倍增加.
- 在糖化豆皮方面表现出高效率,在优化后产生338毫克降解糖/毫升.
- 在棉织物特性方面显著改进,包括粗度,减重和打印能力.
结论:
- 阿斯珀吉勒斯 (Aspergillus awamori) MK788209有效地从农业废物中产生细胞质.
- 生产的细胞酶在豆皮糖化和织应用中非常有效.
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