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量化X射线辐射对1型原蛋白细胞大小尺度粘性弹性的影响

Väinö Mikael Mäntylä¹, Arttu Juhani Lehtonen¹, Vesa Korhonen¹

¹Department of Electrical Engineering and Automation, Aalto University, Espoo FI-02150, Finland.

Journal of biomechanical engineering

|January 6, 2024

概括

高剂量的X射线辐射会使乳腺组织的细胞外基质变软,从而改变其机械性能. 较低的临床剂量显示没有显著变化,这表明放射治疗评估的潜在新方法.

科学领域:

生物物理学的生物物理.
生物材料科学生物材料科学
癌症研究癌症研究

背景情况:

乳腺癌的治疗方法,如乳房造影和放射治疗,使用X射线.
以前的研究主要集中在X射线对乳腺组织细胞的影响上,忽视了细胞外基质 (ECM).
众所周知,ECM的机械特性会影响恶性癌细胞的行为,但X射线对这些特性的影响尚不清楚.

研究的目的:

量化X射线辐射对乳腺组织细胞外矩阵机械性质的影响,以细胞大小的尺度.
研究如何X射线暴露影响矩阵粘弹性,特别是弹性和粘性特征之间的平衡.
开发一种新的技术,用于评估辐射引起的ECM变化.

主要方法:

开发了一种磁性微观学技术来测量矩阵粘弹性.
模拟乳腺组织ECM使用原1型,控制和X射线照射 (50kV管电压).
使用10μm磁探测器,由磁微流体计操纵,以测量探测器位移并推断矩阵粘弹性.

主要成果:

较高的X射线剂量 (320 Gy) 显著软化了原基质,并增加了它们的损失触点,表明了更类似于液体的行为.
临床相关的较低剂量 (54 Gy) 导致矩阵粘性弹性的变化微不足道.
在原基质中观察到粘性弹性的典型空间变化.

结论:

开发的磁性微风学技术可以量化X射线诱导的细胞水平ECM粘性弹性的变化.
高剂量的X射线改变了乳腺组织的ECM机制,可能会影响癌细胞的行为.
这项技术为乳腺癌放射治疗中辐射组织成分的特征提供了一种新的方法.

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