在Phanerochaete chrysosporium中,中央代谢和血生物合成途径之间的途径交叉
Daisuke Miura1, Ryoga Tsurigami2, Hiroyuki Kato2
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan. daisuke.miura@aist.go.jp.
Applied microbiology and biotechnology
|January 6, 2024
概括
研究人员在Phanerochaete chrysosporium中鉴定出血结合蛋白,包括酸盐合成酶和甘甲3-酸盐脱酶. 血红素调节中央代谢和血红素合成途径,影响真菌的芳香降解.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 菌类的新陈代谢 菌类的新陈代谢
背景情况:
- 血红蛋白对各种细胞功能至关重要,包括呼吸和催化.
- 了解像Phanerochaete chrysosporium这样的真菌中的血结合蛋白对于阐明代谢调节至关重要.
- 白色腐烂真菌P. chrysosporium是木质素降解和二次新陈代谢的模型生物.
研究的目的:
- 为了全面调查Phanerochaete chrysosporium中的血结合蛋白.
- 描述血红素与已识别的蛋白质的相互作用,特别是酸盐合成酶 (PcCS) 和甘甲基3-酸盐脱酶 (PcGAPDH).
- 研究血红素在调节中心代谢和血红素生物合成途径中的作用.
主要方法:
- 一种生物化血-斯特雷普塔维丁珠子系统被用于蛋白质测量.
- 进行了复合PcCS和PcGAPDH的异构表达和净化.
- 用光谱分析 (差异光谱) 和酶活性试验来表征血红蛋白相互作用.
- 均质模型被用来预测血红素结合部位.
主要成果:
- 线粒体酸盐合成酶 (PcCS),甘甲基3-酸脱酶 (PcGAPDH) 和一种 thioredoxin 过氧化酶同源被确定为假定的血结合蛋白.
- 黑抑制了PcCS和PcGAPDH活动,这表明其具有调节作用.
- 光谱数据表明,胺残留物可能会在PcCS中结合血红素.
- 同性学建模表明,在乙-CoA结合部位,血红素与PcCS结合.
结论:
- 血红素在P. chrysosporium中的中央代谢和血红素合成途径中充当代谢调节剂.
- 发现了一种新的中央新陈代谢和由血红素介导的血红素生物合成之间的交叉声.
- 这种调节机制对于在真菌芳香降解过程中维持代谢平衡至关重要,影响ATP合成,TCA循环流量和氧化还原平衡.
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