在酵母中高水平生物合成的enantiopure生殖基D
Shubha Sharma1,2, Seema Chaurasia1, Sandeep Dinday1,3
1Plant Biotechnology Division, Council of Scientific and Industrial Research-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, India.
Applied microbiology and biotechnology
|January 6, 2024
概括
酵母中的代谢工程实现了对enantiopure germacrene D (一种香味成分) 的高滴度生物合成. 与以前的方法相比,这种可持续的工艺大大提高了产量.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 格尔马克伦D是香水行业中有价值的基化物,通常在植物精油中以低度发现.
- 目前获得enantiopure生殖基D的方法缺乏可持续和可扩展的供应流程.
研究的目的:
- 开发一种代谢工程化的酵母菌株,用于对enantiopure germacrene D.的高滴度生物合成.
- 建立一个可持续和高效的生产平台,为这种香味化合物.
主要方法:
- 通过整合ergosterol路由酶,改造的Saccharomyces cerevisiae底盘 (CENses5C) 具有增强的Farnesyl Pyrophosphate (FPP) 流量.
- 代码子优化的基因组D合成酶的基因组集成和FPP流量通路的优化.
- 使用ATP-酸酶将线粒体酸盐与细胞酸乙-CoA合.
主要成果:
- 工程酵母菌株实现了 (+) 和 (-) - 胚芽基D的显著更高标位 (与父菌株相比增加了多达120倍).
- 优化菌株 (CENses8(+D) *和CENses8(-D) *) 在摇瓶发酵中产生高达2519.46μg/ml的基基基D.
- 迄今为止,在摇瓶发酵中,已经证明了 enantiopure germacrene D 的最高标位.
结论:
- 糖菌 (Saccharomyces cerevisiae) 的代谢工程是一种可行的策略,可以生产高度的enantiopure germacrene D.
- 开发的酵母平台提供了一个可持续和可扩展的替代品为生殖基D供应.
- 这一进步解决了对香料成分高效工业生产的需求.
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