在上皮细胞入侵期间,Shigella会产生独特的IAM亚群,以促进有效的细胞内形成
Lisa Sanchez1, Arthur Lensen1, Michael G Connor2
1Institut Pasteur, Université Paris Cité, CNRS UMR3691, Dynamics of Host-Pathogen Interactions Unit, 75015 Paris, France.
European journal of cell biology
|January 6, 2024
概括
石格拉柔性菌的入侵涉及独特的囊泡,称为感染相关的巨胞体 (IAMs). 排序Nexin-8 (SNX8) 控制了含有细菌的真空细胞 (BCV) 破裂和逃入宿主细胞细胞质的时间.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 传染性疾病 传染性疾病
背景情况:
- 希格拉柔性菌通过3型分泌系统入侵宿主细胞,形成含有细菌的真空细胞 (BCV).
- 感染相关的巨细胞 (IAMs) 在侵袭期间形成,对于BCV逃脱至关重要,但潜在的分子机制仍然不清楚.
研究的目的:
- 为了确定参与细菌含有真空体 (BCV) 在Shigella flexneri入侵期间拆卸的宿主因素.
- 阐明Sorting Nexin-8 (SNX8) 在调节BCV逃生中的作用.
主要方法:
- 基于显微镜的屏幕用于识别招募到IAM的BAR域含有蛋白质.
- 研究了PI(3) P和RAB11A在IAM亚群中的作用.
- 利用siRNA消耗SNX8并评估其对BCV分解的影响.
主要成果:
- 鉴定了Sorting Nexin-8 (SNX8) 作为一个PI(3) P依赖的蛋白质,在BCV拆卸之前被招募到IAM中.
- 发现了两个不同的IAM亚群,一个涉及SNX8,另一个涉及RAB11A,影响BCV破裂.
- 抑制PI(3) P或SNX8功能延迟了BCV破裂和逃逸.
结论:
- 在控制BCV破裂的时间和细菌逃入宿主细胞质中时,SNX8起着至关重要的作用.
- 石格拉会颠覆由SNX8调节的特定IAM亚群,以建立其细胞内.
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