通过α/β酸酶RsbQ通过Bacillus subtilis感知丁化物
Kim T Melville1, Muhammad Kamran1, Jiaren Yao1
1School of Molecular Sciences, The University of Western Australia, Perth WA 6009, Australia.
Current biology : CB
|January 6, 2024
概括
细菌的RsbQ蛋白感知到植物的布丁化物,作为酶. 这些化合物调节细菌生长和生物膜的形成,这表明植物微生物相互作用中的跨王国信号.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 植物科学 植物科学
背景情况:
- 小分子调节所有生命领域的关键生物过程.
- 在植物中,诸如strigolactones和karrikins之类的丁化物是影响发育和共生相互作用的生长调节剂.
- DWARF14 (D14) 和KARRIKIN INSENSITIVE2 (KAI2) 是这些分子的植物酶受体,需要酶活性进行信号传递.
研究的目的:
- 阐明RsbQ的未知分子功能,RsbQ是Bacillus subtilis中发现的D14和KAI2的同类.
- 为了研究RsbQ与植物衍生的布丁化物化合物的相互作用.
- 了解丁化物在细菌生长调节和跨王国信号传递中的作用.
主要方法:
- 热稳定性测试使用合成格拉克GR24及其衍生物dGR24.
- 生物化学测定以确认RSbQ的酶活性和共价变异.
- 在体内实验中,将GR24和dGR24应用于Bacillus subtilis,以评估信号抑制和基因表达变化.
- 显微镜观察对细菌生长过渡 (从浮游生物到生物膜) 的影响.
主要成果:
- RsbQ被GR24和dGR24破坏了稳定,这表明对这些植物芽化物的感知.
- RsbQ表现出功能性布丁化物酶活性,类似于植物D14和KAI2.2.
- 外源GR24和dGR24在体内抑制了RsbQ的信号传递,而dGR24更强效.
- dGR24应用模拟了rsbQ突变,降低了SigmaB调节的转录,并促进了生物膜的形成.
结论:
- RsbQ 作为一个布丁化酶,对植物衍生信号分子作出反应.
- 细菌的布诺化物感知影响生长反应和生物膜的发展.
- 巴特诺利德可以作为介导植物-细菌沟通的跨王国信号,在树叶球中起作用.
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