来自PTEN的CircPTEN-MT调节了线粒体能量代谢
Danhui Ruan1, Jiancheng Xu1, Yang Liu1
1Institute of Systems Biomedicine, Department of Pathology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University International Cancer Institute, Peking-Tsinghua Center of Life Sciences, Peking University Health Science Center, Beijing 100191, China.
Journal of genetics and genomics = Yi chuan xue bao
|January 6, 2024
概括
一种新型的循环RNA,circPTEN-MT,通过与LRPPRC相互作用来调节线粒体能量代谢. 这一发现揭示了PTEN超出其线性形式的新功能,影响细胞能量生产.
科学领域:
- 线粒体生物学 线粒体生物学
- RNA生物学的RNA生物学
- 基因调节 基因调节
背景情况:
- 酸酶和张素同类基因 (PTEN) 是细胞过程中的关键基因.
- 循环RNAs (circRNAs) 是基因表达的新兴调节者.
- 与PTEN相关的circRNAs的作用,特别是在线粒体中,在很大程度上是未被探索的.
研究的目的:
- 为了识别和描述PTEN相关的circRNAs.
- 研究circPTEN-MT在线粒体能量代谢中的功能.
- 为了阐明circPTEN-MT作用的分子机制.
主要方法:
- 来自PTEN前列3-5.5的circPTEN-MT (hsa_circ_0002934) 的识别方法
- 在线粒体内研究circPTEN-MT的局部化.
- 研究circPTEN-MT与LRPPRC和SLIRP的相互作用.
- 评估线粒体mRNA多化和复杂I亚单元水平.
- 测量线粒体膜潜力和ATP的产生.
主要成果:
- 环PTEN-MT局部存在于线粒体中,并与LRPPRC相互作用.
- 破坏circPTEN-MT会破坏LRPPRC-SLIRP的相互作用,并抑制线粒体mRNA多基化.
- 在circPTEN-MT敲除后观察到线粒体复合物I亚单元mRNA的水平降低,线粒体膜潜能降低,ATP生产受损.
- CircPTEN-MT作为线粒体能量代谢的关键调节剂.
结论:
- 环PTEN-MT是一种来自PTEN的新型线粒体循环RNA.
- 在调节线粒体功能和细胞能量生产方面,CircPTEN-MT起着至关重要的作用.
- 这项研究突出了PTEN基因产生的线性和圆形RNA的独立和独特功能.
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