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从临床样本中绝对量化结合抗体

Chan Tang¹, Annemiek Verwilligen¹, Jerald Sadoff¹

¹Janssen Vaccines & Prevention, Leiden, The Netherlands.

|January 6, 2024

概括

我们开发了MASCALE,这是一种用于绝对抗体量化的质谱法. 这种方法对ELISA参考血清进行校准,使不同测试和设置中免疫反应的准确比较成为可能.

科学领域:

免疫学免疫学免疫学
分析化学分析化学
生物技术是生物技术.

背景情况:

准确量化抗体反应对于疫苗和治疗开发至关重要.
当前的方法通常依赖于任意的参考值,限制了交叉测试的可比性.
标准化参考材料对于可靠测量免疫反应至关重要.

研究的目的:

开发一种可通用的方法来绝对量化抗体.
克服在不同测定和实验室中比较抗体反应的局限性.
在临床试验中提供一种更准确,更可靠的方式来解释免疫反应.

主要方法:

开发了MASCALE (质谱法使转换到绝对水平的ELISA抗体).
校准的酶相关免疫吸收试验 (ELISA) 参考血清使用质谱学.
使用蛋白型作为人类IgG的代理,将任意的ELISA单位转换为绝对数量.

主要成果:

建立了一种使用质谱法绝对量化抗体的方法.
在两个实验室之间成功比较了结合性测试.
由试验性HIV-1疫苗诱导的免疫反应的评估跨幅大小宽度.

结论:

马斯卡尔为绝对抗体定量提供了强大的解决方案.
该方法增强了在临床环境中对免疫反应的解释.
MASCALE扩大了在不同实验条件下比较免疫反应的选项.

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相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.