电机构成交换机和丁糖在细菌吸收营养中的作用
Maximilian Zinke1, Maylis Lejeune1, Ariel Mechaly2
1Institut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, F-75015, Paris, France.
Nature communications
|January 6, 2024
概括
格拉姆阴性细菌使用系统来吸收营养. 我们发现ExbD.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 格拉姆阴性细菌需要通过系统进行活性营养物质的运输.
- 系统利用质子驱动力来通过膜进口营养物质.
- 在系统中,精确的能量转移机制尚未完全被理解.
研究的目的:
- 调查ExbD在系统中的周等离子域的作用.
- 阐明格拉姆阴性细菌对营养吸收的能量转移机制.
- 为系统提出了一种机械模型.
主要方法:
- 在体内表型测定.
- 对ExbD周等离子体域的结构分析.
- 保存机制的序列分析.
主要成果:
- 在ExbD周等离子域的功能作为一个动态二元体,在开放和关闭状态之间切换.
- 这种形状交换机对于细菌的营养吸收至关重要.
- 在TonB中,ExbD的开放状态诱导了从混乱到秩序的过渡,促进了能量传输.
结论:
- 提出了系统的机械模型,突出了ExbD的双重作用和二糖的催化功能.
- 已识别的机制在其他细菌系统中得到保存.
- 这项研究为新型抗菌策略提供了潜在的目标.
相关概念视频
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Microtubule Associated Motor Proteins
8.0K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
8.0K
Bacterial Signaling
32.2K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.2K
Prokaryotic Transcriptional Activators and Repressors
21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.1K
Mechanical Protein Functions
4.9K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
4.9K
Structure of Porins
3.0K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
3.0K


