从发酵饮料中分离出来的酵母菌株产生具有抗炎作用的细胞外囊泡
Stefano Nenciarini1, Roberta Amoriello2, Giovanni Bacci1
1Department of Biology, University of Florence, Florence, Italy.
Scientific reports
|January 6, 2024
概括
益生菌酵母释放细胞外囊泡 (EVs),可以通过调节免疫细胞来减少炎症. 这些来自发酵牛奶的酵母EV可能解释了益生菌食品的一些抗炎作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 细胞外囊泡 (EVs) 对于细胞间通信至关重要,它们携带诸如核酸,脂质和蛋白质等生物分子.
- 已知真菌EVs调节宿主免疫反应,主要研究在感染环境中.
- 在其他与健康相关的环境中,来自非致病酵母的EVs的作用仍未得到充分研究.
研究的目的:
- 从益生菌发酵牛奶中分离出来的Saccharomyces cerevisiae和Pichia fermentans中的EV的特征.
- 在体外研究这些酵母EVs对人类免疫细胞的免疫调节作用.
- 在益生菌食品消费的背景下,探索酵母EVs介导的潜在抗炎机制.
主要方法:
- 从选定的酵母菌株中分离和描述EVs.
- 在体外免疫试验中使用人类单细胞衍生的树突细胞.
- 对EV中小RNA含量的分析,重点关注mRNA序列.
主要成果:
- 来自发酵牛奶的酵母EV显著降低了树突细胞中的抗原呈现活性.
- EVs的小RNA部分主要由酵母mRNA组成.
- 来自不同酵母菌种的EV丰富了特定的分子功能.
结论:
- 来自益生菌发酵牛奶的酵母衍生的EV具有免疫调节性质.
- 这些EV可以抑制树突细胞抗原的呈现,这表明它具有抗炎作用.
- 人类免疫细胞和酵母EV之间的相互作用可能有助于益生菌食品的健康益处.
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