相关实验视频
Updated: Jul 6, 2025

08:05
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Published on: November 6, 2021
4.4K
果Ca2+/H+交换器MdCAX2L-2在调节Ba2+耐受性方面具有积极的功能
Chuang Mei1, Peng Yan1, Beibei Feng1
1The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China.
Plant physiology and biochemistry : PPB
|January 7, 2024
概括
果果果果果果果是什么意思
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 对于植物生长和应激反应至关重要.
- 土壤中的重金属降低了作物产量和质量.
- /质子 (H+) 交换器 (CAX) 运输Ca2+和金属离子,调节植物发育和应力耐受性.
研究的目的:
- 为了克隆和表征一个果CAX正义词,MdCAX2L-2.
- 研究MdCAX2L-2在植物对重金属,特别是 (Ba2+) 的耐受性方面的作用.
主要方法:
- 在果中克隆MdCAX2L-2.
- 在各种果组织和Ca2+和Ba2+处理下进行表达分析.
- 在植物和酵母细胞中的亚细胞局部化研究.
- 通过酵母突变K667和转基因Arabidopsis的过度表达的功能分析.
- 在BaCl2压力下进行的表型和生理评估.
主要成果:
- MdCAX2L-2是由Ca2+和Ba2+构成性表达和诱导的.
- 蛋白质定位在真空膜上.
- 过度表达MdCAX2L-2使酵母对酵母中高的Ca2+和Ba2+产生耐受性.
- MdCAX2L-2通过促进真空封存和减少反应性氧物种 (ROS) 来增强Arabidopsis和果的Ba2+耐受性.
结论:
- MdCAX2L-2在增强植物对Ba2+压力的耐受性方面发挥着重要作用.
- 这种传送器的功能是将Ba2+隔离到真空中,并减轻ROS积累.
- 这些发现提供了关于CAX蛋白在水果作物耐压力作用的见解.
相关概念视频
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.3K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.3K
The Role of Ion Channels in Neuronal Computation
3.2K
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
3.2K

