针对Streptococcus pneumoniae的Alpha-Enolase的来自的抗体的表征
Chi-Hsin Lee1, Chao-Jung Wu1, Yi-Yuan Yang2
1School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110301, Taiwan; Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110301, Taiwan.
International immunopharmacology
|January 7, 2024
概括
研究人员开发了针对Streptococcus pneumoniae alpha-enolase (spEno1) 的新型单链可变片段 (scFv) 抗体. 这些抗体显示出针对肺炎球菌感染的免疫治疗策略的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 肺炎链球菌 (Streptococcus pneumoniae) 会导致严重的感染,如肺炎和脑膜炎.
- 肺炎菌中的抗菌素耐药性是一个日益严重的全球健康问题.
- 针对像α-enolase (spEno1) 这样的表面蛋白提供了替代治疗策略.
研究的目的:
- 开发针对S. pneumoniae alpha-enolase (spEno1) 的新型单克隆抗体.
- 评估这些抗体在抑制细菌毒性因子方面的有效性.
主要方法:
- 重组spEno1蛋白净化和免疫用于IgY抗体生成.
- 使用菌体显示技术构建和选单链可变片段 (scFv) 抗体库.
- 评估抗体结合活性,表位图绘制和抑制试验.
主要成果:
- 对spEno1的十种scFv抗体被成功识别和表征.
- 这些scFv抗体表现出与spEno1.1的特定结合.
- 这些抗体有效地抑制了spEno1和人类等离子体之间的相互作用.
结论:
- 生成的scFv抗体显示出作为一种潜在的免疫治疗剂对抗S. pneumoniae的潜力.
- 向spEno1代表了对抗肺炎球菌感染的可行策略,特别是在抗生素耐药性增加的背景下.
相关概念视频
01:10Antibody Structure
60.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
60.2K
01:25Antibody Structure and Classes
915
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
915