Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

13.5K
In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
13.5K
Fates of Pyruvate01:20

Fates of Pyruvate

8.5K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.5K
What is Glycolysis?00:56

What is Glycolysis?

165.0K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
165.0K
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

163.6K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
163.6K
Reactions of Aldehydes and Ketones: Baeyer–Villiger Oxidation01:22

Reactions of Aldehydes and Ketones: Baeyer–Villiger Oxidation

4.2K
Baeyer–Villiger oxidation converts aldehydes to carboxylic acids and ketones to esters. The reaction uses peroxy acids or peracids and is often catalyzed by acid. The reaction is named after its pioneers, Adolf von Baeyer and Victor Villiger. The reaction is achieved by a wide range of peracids such as m-chloroperoxybenzoic acid (mCPBA), perbenzoic acid (C6H5COOOH), peracetic acid (CH3COOOH), hydrogen peroxide (H2O2), and tert-butyl hydroperoxide (t-BuOOH).
The carbonyl center is...
4.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Biodegradability of Acrylate-Lipoic Acid Copolymers.

Journal of the American Chemical Society·2026
Same author

Orthogonal quorum sensing circuits enable dynamic regulation in Escherichia coli.

Metabolic engineering·2026
Same author

Engineered Gram-Positive Based Quorum Sensing for Metabolic Control in <i>Escherichia coli</i>.

ACS synthetic biology·2025
Same author

Deciphering allosterism of an <i>Escherichia coli</i> hexuronate metabolism regulator: UxuR.

RSC medicinal chemistry·2025
Same author

Designing for degradation: the importance of considering biotic and abiotic polymer degradation.

Environmental science. Processes & impacts·2025
Same author

α-Substituted 3-hydroxy acid production from glucose in Escherichia coli.

Metabolic engineering·2024

相关实验视频

Updated: Jul 6, 2025

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.0K

来自可变混合基质的D-糖酸盐的一致生物合成.

Cynthia Ni1, Kristala L J Prather1

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Metabolic engineering
|January 7, 2024
PubMed
概括

代谢工程师现在可以使用混合废料原料进行微生物生物合成. 这种新方法确保了从复杂的基质中产生一致的D-糖酸盐,克服了以前的挑战.

科学领域:

  • 代谢工程是代谢工程.
  • 合成生物学 合成生物学
  • 生物技术是生物技术.

背景情况:

  • 微生物生物合成传统上使用单一的,纯化的基质.
  • 废物流提供可再生原料,但由于混合和不断变化的成分,存在挑战.
  • 由于表达多个酶的代谢负担,阻碍了复杂原料的高效转化.

研究的目的:

  • 开发一种使用混合废物原料进行微生物生物合成的可靠方法.
  • 为了使多种基质从多种基质中产生一致的D-糖酸盐.
  • 展示一种用于将复杂原料转化为有价值产品的新方法.

主要方法:

  • 由特定基质激活的可诱导生物合成途径的工程微生物.
  • 利用两条新的途径将银酸盐和葡萄酸盐转化为D-糖酸盐.
  • 使用单基板和混合基板料进行发酵,以评估产品标位.

主要成果:

  • 达到了1.8 ± 0.3gL-1的D-糖酸盐标位从银酸盐和1.64 ± 0.09gL-1从葡萄糖酸盐.
  • 混合基质发酵产生了D-糖酸位从1.48 ± 0.03到1.8 ± 0.1gL-1.1之间.
  • 在单基质发酵和混合基质发酵之间没有观察到D-糖位的显著差异.

更多相关视频

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

8.0K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.8K

相关实验视频

Last Updated: Jul 6, 2025

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.0K
Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

8.0K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.8K

结论:

  • 从单基质和混合基质表现出一致的D-糖生物合成.
  • 通过基质可诱导途径验证了一种可靠的方法来转化复杂的原料.
  • 这种方法为从废物流中可持续生产微生物提供了一个有希望的战略.