用皮质醇生物合成抑制剂治疗的人类上腺皮层NCI-H295R细胞的转录组数据
Soo Hyun Kim1, Hyun Jung Kim2, Jong-Wha Jung3
1Department of Brain and Cognitive Sciences, Scranton College, Ewha Womans University, Seoul 03760, Republic of Korea.
Data in brief
|January 8, 2024
概括
像CJ28这样的新型氏酸盐衍生物通过影响类固醇生成和胆固醇生物合成,有效抑制皮质醇的产生. 这项研究为了解上腺皮质基因表达网络提供了有价值的转录基因数据.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 上腺皮质类固醇生物合成失调会导致诸如库辛综合征之类的疾病.
- 目前的皮质醇过度产生的治疗方法在有效性和耐受性方面存在局限性.
- 需要新的抑制剂来有效地解决皮质醇过度产生的问题.
研究的目的:
- 呈现与新型皮质醇生物合成抑制剂CJ28或 metyrapone 治疗的上腺细胞的转录组数据.
- 为了研究基因表达的变化,底层的上腺皮质类固醇生成.
- 为了解皮质醇抑制的分子机制提供资源.
主要方法:
- 用载体,CJ28 (30微米) 或 metyrapone (30微米) 治疗NCI-H295R细胞24小时.
- 总RNA被提取并在Illumina NovaSeq 6000平台上进行配对末端测序.
- 转录组数据被分析,以确定基因表达的变化.
主要成果:
- CJ28显示显著抑制基底和刺激皮质醇的产生.
- CJ28的抑制作用与减弱的类固醇生成和胆固醇生物合成有关.
- 转录组分析显示,对CJ28和 metyrapone 的反应有不同的基因表达特征.
结论:
- 由CJ28举例说明的氏醇基氨酸衍生物,代表了一类有前途的皮质醇生物合成抑制剂.
- 提供的RNA-seq数据提供了对这些抑制剂影响的分子通路的见解.
- 这一数据集是未来对上皮功能和治疗开发的研究的宝贵资源.
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