转录激活剂XYR1的结构指导工程,用于在Trichoderma reesei中无诱导剂生成红细胞解酶
Qinqin Zhao1, Zezheng Yang2, Ziyang Xiao1
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.
Synthetic and systems biotechnology
|January 8, 2024
概括
通过将氨酸873转化为氨酸来改造Trichoderma reesei XYR1蛋白质,工程显著提高了红细胞分解酶的产量. 这一突破提升了生物质中的葡萄糖和西的产量,为工业酶生产提供了一种新的战略.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- 特里科德尔马 (Trichoderma reesei) 是工业细胞分解酶生产的关键真菌.
- 在T. reesei.中,XYR1是细胞酶和半细胞酶基因的主要激活剂.
- 缺乏结构信息阻碍了XYR1.1.的合理工程.
研究的目的:
- 确定XYR1中的新工程目标,以增强酶生产.
- 为了研究特定的XYR1突变对林氏细胞分解酶产量的影响.
- 使用工程T. reesei菌株提高生物质转化效率.
主要方法:
- 使用AlphaFold2.2.使用XYR1的结构预测.
- 在 alanine 873.3 的 XYR1 的位点定向突变发生.
- 用不同的碳来源 (纤维素,葡萄糖) 来发酵工程T. reesei菌株.
- 酶活性测定和生物质糖化分析.
主要成果:
- 氨酸873转化为氨酸 (A873Y) 的突变显著增强了溶酶的产生.
- 在葡萄糖中,西兰酶和蜂酶的产生分别增加了56.7倍和3.3倍.
- 与V821F突变相比,A873Y突变显示出更高的性能.
- 来自A873Y菌株的酶从预处理的玉米中产生了51%更多的葡萄糖和52%更多的糖.
结论:
- 氨酸873是工程XYR1.1.的新和有效的目标.
- 该A873Y突变提供了一个有前途的策略,以促进T. reesei.的纤维细胞分解酶生产.
- 这项研究有助于理解XYR1调节和提高工业酶能力.
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