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重建自细胞膜结合的结果显示,Atg11可以在货物模拟物上结合和聚合囊泡

Devika Andhare¹, Sarah Katzenell¹, Sarah I Najera²

¹Department of Chemistry, Dartmouth College, Hanover, NH 03755, USA.

bioRxiv : the preprint server for biology

|January 8, 2024

概括

自蛋白Atg11与膜结合,从而启动线粒体自. 这种依赖于膜曲率和特定螺旋的结合,对于酵母菌中的线粒细胞衰变至关重要.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生化学

背景情况:

自是一种基本的细胞过程,用于降解受损的细胞器官,包括线粒体.
线粒体自 (线粒体自) 对于细胞健康至关重要,并且在各个物种中得到保护.
在酵母体中,线粒体细胞衰变的启动涉及线粒体中的Atg11和Atg32蛋白的相互作用.

研究的目的:

调查选择性自性支架蛋白Atg11在线粒细胞衰变的启动中的作用.
阐明Atg11与自细胞膜相互作用的机制.
了解Atg11-Atg32相互作用如何有助于自细胞囊泡到线粒体的招募.

主要方法:

在体外结合测试以评估Atg11与脂质膜的相互作用.
位点定向的突变发生,以删除Atg11中预测的两螺旋体.
使用模仿线粒体外膜组成的巨型单状囊泡进行生化分析.
显微镜观察酵母菌中线粒开始部位.

主要成果:

在实验室中,Atg11通过两螺旋通过曲率依赖的方式与自体样膜结合.
在Atg11中删除两螺旋,延迟了线粒开始部位的形成.
Atg11-Atg32的相互作用促进了自细胞样囊泡的结合,以模仿线粒体膜.

结论:

Atg11结合自细胞膜的能力对于启动线粒细胞衰变至关重要.
膜曲率和Atg11的两螺旋在这种结合中起着关键作用.
Atg11-Atg32复合体充当支架,将自细胞和线粒体聚集在一起进行降解.

关键词:

生物化学生物化学线粒细胞衰变 (mitophagy) 是一种神经衰变的过程.复制复制复制选择性自自是一种选择性自.酵母酵母是一种酵母.

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