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Updated: Jul 6, 2025

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2
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Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Published on: September 18, 2021

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多样样单细胞RNA-seq数据的差异检测工作流程

Jeroen Gilis1,2,3,4, Laura Perin1,5, Milan Malfait2

  • 1These authors contributed equally.

bioRxiv : the preprint server for biology
|January 8, 2024
PubMed
概括

这项研究引入了单细胞转录组的差异检测 (DD) 分析,补充了传统的差异基因表达 (DE) 分析. DD识别了具有改变检测率的基因,为基因调节和细胞功能提供了新的见解.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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