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对vanD抗菌素Enterococcus faecium的出现进行基因组研究

Sarah L Baines¹, Romain Guérillot¹, Susan Ballard²

¹Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.

Access microbiology

|January 8, 2024

概括

一种新型的vanD vancomycin-resistant Enterococcus faecium (VREfm) 通过获得携带vanD基因的移动遗传元件,从一个易受感染的菌株中出现. 这个元素在全球范围内传播,可以在细菌属之间转移,突出显示了抗菌素耐药性的潜在传播机制.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

抗万通菌素的肠球菌 (VRE) 是人类感染的重要原因,主要是由于vanA和vanB基因型.
关于其他VRE基因型的出现和传播的知识有限,例如vanD.

研究的目的:

从基因组学上鉴定出一种易受菌素的E. faecium (VSEfm) 菌株中出现的vanD VRE faecium (VREfm) 的特征.
研究涉及VREfm发展的基因组变异和移动遗传元素.
将vanD VREfm与全球vanD-harboring孤立群进行比较,以了解传播模式.

主要方法:

长读全基因组测序和VSEfm和VREfm分离物的基因组闭包.
整基因组对齐,交叉映射和正义基因组聚类以确定基因组差异.
遗传学分析和与全球收集的vanD-harboringEnterococcus基因组进行比较.

主要成果:

该VREfm获得了一个142.6kb的整合性结合元件 (ICE),其中包含vanD位点.
原生结合酶基因 (ddl) 由于IS Efm1插入而被破坏.
在VREfm中发生了1.74 Mb大的染色体逆转.
基因组比较揭示了vanD位点周围的一个保存区域,这表明在多种隔离物中存在一个共同的移动元素和集成位点.
有证据表明,假定的ICE在全球范围内传播,并且可以在不同细菌系中转移.

结论:

范德VREfm通过获得全球传播的整合性结合元件而再次出现.
这种ICE促进了VanD耐药性的传播,并且可以被各种细菌种类获得.
了解vanD VREfm的出现和传播对于打击抗菌素耐药性至关重要.

关键词:

肠球菌 (Enterococcus) 是一个细菌.进行比较的基因组学.整合性的结合元素.范D 范D 范D 的意思范科米辛的耐药性

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