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在严重和重症流感患者之间比较唾液微生物群特征
Zhixia Gu1,2,3,4, Yuanyuan Zhang1,2,3,4, Xue Zhao1
1Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Frontiers in cellular and infection microbiology
|January 8, 2024
概括
这项研究揭示了严重的流感A病毒 (IAV) 感染中明显的下呼吸道 (LRT) 微生物变化. 危急病患者的细菌体数量减少,严重病例的尼塞里亚和菌体数量增加.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 呼吸系统医学 呼吸系统医学
背景情况:
- 有限的研究存在于下呼吸道 (LRT) 微生物群的变化后流感A病毒 (IAV) 感染.
- 临床队列为了解这些微生物转变提供了有价值的起点.
研究的目的:
- 为了比较分析液微生物群的多样性和组成,重症与重症流感患者.
- 为了确定与疾病严重程度相关的特定细菌种群.
主要方法:
- 使用16S-rDNA测序分析了64名感染IAV患者 (48名严重,16名危急病) 的唾液微生物群.
- 线性差异分析效果大小 (LEfSe) 确定了分类学差异.
- 斯皮尔曼相关性分析了微生物群,病毒载量 (Ct值) 和炎症标志物 (CRP) 之间的关联.
主要成果:
- 危急病患者的细菌体显著减少 (p=0.031).
- 严重的流感病例显示出尼塞里亚菌,波菲罗莫纳斯菌,阿基诺菌,阿洛普雷沃特拉菌,TM7x菌和克洛斯特里迪亚菌的过度代表性_UCG-014.
- 艾洛普雷沃特拉与流感Ct值相反相关;CRP与海莫菲勒斯和波菲罗莫纳斯有正相关.
结论:
- 这些发现突出了严重和危急性流感患者之间LRT微生物群组成的显著差异.
- 需要进一步研究,以了解特定细菌 (如Alloprevotella) 在IAV病原和疾病进展中的作用.
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