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最近收集的一种Xanthomonas透光隔离物编码TAL效应物,与较旧,不那么有毒的隔离物不同

Diego E Gutierrez-Castillo¹, Emma Barrett¹, Robyn Roberts¹

¹Department of Agricultural Biology, Colorado State University, Fort Collins, CO, USA.

Microbial genomics

|January 8, 2024

概括

较新的Xanthomonas translucens分离物导致谷物中的细菌叶条菌病,由于遗传变化,特别是转录激活器类效应物,更具侵略性,解释了增加的毒性.

科学领域:

植物病理学植物病理学
微生物基因组学微生物基因组学
农业科学农业科学

背景情况:

克桑托莫纳斯透光会导致谷物中的细菌叶条 (BLS),这是一个重新出现并越来越具有破坏性的植物病.
事证据表明,最近的X. translucens分离物可能比较古老的菌株具有更高的毒性.

研究的目的:

调查最近在科罗拉多州X. translucens分离物中观察到的攻击性增加的遗传基础.
将最近分离的基因组与较老的菌株进行比较,以确定导致毒性的遗传变异.

主要方法:

两种新分离物 (CO236,CO237) 的表型表征和病态表征名称 (Xtt,Xtu).
使用牛津纳米孔长读技术进行全基因组测序.
基因组比较分析专注于III型和转录激活剂类效应因子.

主要成果:

确认了CO236 (Xtt) 和CO237 (Xtu) 隔离物,与参考基因组相比,基因组结构没有重大差异.
在Xtu CO237中,Xtu隔离物中保留了III型因子.
在Xtu CO237中发现了转录激活器样效应因子的显著变异,与增加的毒性相关.

结论:

转录激活器样效应因子的差异可能是最近X. translucens分离物的增强毒性.
了解这些遗传因素对于管理谷物中重新出现的BLS疾病至关重要.

关键词:

克桑托蒙纳斯 (Xanthomonas) 是一种细菌基因组学是一种细菌基因组学.大麦大麦大麦大麦大麦大麦大麦大麦效应器效应器是一个效应器.长时间阅读序列排序.植物免疫力植物免疫力植物微生物相互作用毒性毒性是一种毒性.小麦小麦小麦小麦小麦小麦小麦.

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