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Updated: Jul 6, 2025

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Taste Exam: A Brief and Validated Test
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苦味受体与类似于ACE2的SARS-CoV-2-spike 1蛋白建立了稳定的结合亲和力
Senthil Arun Kumar1, C Selvaa Kumar2, Norine Dsouza3
1Department of Biotechnology, Parul Institute of Technology, Parul University, Vadodara, Gujarat, India.
Journal of biomolecular structure & dynamics
|January 8, 2024
概括
与野生型菌株不同的是,SARS-CoV-2三角形变体对苦味受体的结合减少. 这解释了为什么三角洲感染不会引起早期COVID-19变种常见的味觉功能障碍.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 计算生物学是一种计算生物学.
背景情况:
- 由SARS-CoV-2引起的COVID-19导致了全球显著的死亡率.
- 味觉功能障碍 (味觉丧失) 是野生型SARS-CoV-2感染的常见症状.
- 在感染SARS-CoV-2三角体变异的患者中,口味功能障碍的缺失尚未得到充分了解.
研究的目的:
- 为了研究SARS-CoV-2变体和苦味受体之间的分子相互作用.
- 阐明野生型和三角洲型变体之间味觉功能障碍差异的原因.
- 评估苦味受体 (T2Rs) 与野生型和三角形SARS-CoV-2的尖端蛋白受体结合域 (RBD) 的结合亲和力.
主要方法:
- 在基分子对接和结合能量的计算 (MM/PBSA).
- 从野生型和三角形SARS-CoV-2变体中评估S1蛋白的受体结合域 (RBD) 之间的相互作用.
- 对关键苦味受体的结合 afinities 的评估 (T2R-4,10,14,19,31,38,43,46).
主要成果:
- 野生型的SARS-CoV-2 RBD与ACE2相比,与T2R-46具有更强的结合亲和力.
- 德尔塔变异突变 (mut1, mut2) 对大多数苦味受体的结合明显较弱.
- 一个值得注意的例外是T2R-43与delta变异mut1的相互作用.
结论:
- 德尔塔变异对苦味受体的结合亲和力降低解释了缺乏味觉功能障碍.
- 野生型变种与T2R-46的更强的相互作用可能导致其毒性和系统性影响.
- 提议T2R-46作为野生型SARS-CoV-2的潜在替代结合标,需要进一步的实验验证.
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