使用WAX HILIC-MS对氏丁硫酸盐进行分糖化合物组合分析,附有前列前胺胺标签;在孕前症中对胎盘施加
Imeobong U Antia1, Frank A Hills1, Ajit J Shah1
1Glycan Research Group, Department of Natural Sciences, Faculty of Science and Technology, Middlesex University, London, UK. A.J.Shah@mdx.ac.uk.
Analytical methods : advancing methods and applications
|January 8, 2024
概括
胎盘中的里硫酸盐 (CS) 组成在预先怀孕和正常怀孕之间有所不同. 这一发现表明CS分析可能有助于诊断妊娠障碍和理解分娩.
科学领域:
- 生物化学 生物化学
- 产科 产科 产科 产科 产科
- 分析化学 分析化学
背景情况:
- 氏丁硫酸盐 (CS) 和皮肤硫酸盐是参与细胞信号传输的糖氨基甘油 (GAG).
- 在胎盘中,CS的表达很高,其蛋白质甘油蛋白 (decorin) 在子宫前和胎儿生长限制下降.
- 改变的GAG链组成可能会影响生殖前的细胞过程.
研究的目的:
- 开发一种LC-MS方法,用于CS分糖化合物的组合分析,具有前列前胺胺标签.
- 为了研究胎盘CS分糖化合物组成在孕前的变化.
- 探索CS分析用于妊娠障碍的诊断潜力.
主要方法:
- 开发一种液体色谱-质谱法 (LC-MS) 方法.
- 使用procainamide标签的前列衍生.
- 从胎盘组织中提取的ondroitin硫酸盐的分糖化合物组成分析.
主要成果:
- 该研究成功开发了一种LC-MS方法用于CS脱糖化分析.
- 在子宫前和其他怀孕条件之间观察到胎盘CS的不同分糖化合物组成.
- CS硫化可能在产妇分娩中发挥重要作用.
结论:
- 开发的LC-MS方法可以在各种妊娠条件下区分CS组成.
- 胎盘CS分糖化合物组成在妊娠前中发生变化,这表明有诊断潜力.
- 酸硫化与孕产妇分娩的机制有关.
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