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哺乳动物细胞的神经和肌肉分化伴随着PHF10异形表达的变化

D O Bayramova^1,2, A M Azieva³, A V Feoktistov^1,2

¹Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

Doklady. Biochemistry and biophysics

|January 8, 2024

概括

在PBAF染色体重塑复杂的复杂.

科学领域:

生物化学生物化学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

作为SWI/SNF家族成员的PBAF染色体重塑复合体,对于发育过程中的基因表达调节至关重要.
在PBAF复合体内,特定的子单元具有与基因素N端序列结合的域,这决定了染色质结合的特异性.
PHF10是一个关键的PBAF子单元,具有用于染色体相互作用的独特DPF域,另一个缺少该域的替代异型也存在.

研究的目的:

调查PHF10异型在人类和小鼠细胞中神经元和肌肉分化过程中的表达动态.
确定PHF10异型表达的变化是否会影响PBAF复合体在分化过程中对基因调节的作用.

主要方法:

在人体和小鼠细胞系的体外分化过程中分析PHF10异型表达模式.
在分化诱导之前和之后对PHF10异形丰度的比较评估.

主要成果:

在神经元和肌肉分化过程中观察到PHF10异型表达的显著转变.
缺乏DPF域的PHF10异型随着分化进展,越来越多地取代含有DPF的异型.
这种异形开关表明,在细胞分化过程中,PBAF复合体功能可能发生改变.

结论:

在分化过程中PHF10异型的差异表达表明了涉及PBAF复合体的调节机制.
PHF10异型组合的变化可能会调节PBAF复合体在分化细胞中的基因选择性.
这一发现为细微调整组织发育所必需的基因表达程序提供了洞察力.

关键词:

在PBAF中,PBAF是PBAF.在 PHF10 中,PHF10 是在 SWI/SNF 中.染色体重塑染色体重塑的方法基因表达的基因表达方式神经分化的神经分化.

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