邻近的 lncRNAs MALAT1 和 NEAT1 的深度保护和意想不到的进化历史
Forrest Weghorst1, Martí Torres Marcén1, Garrison Faridi1
1Department of Neurobiology and Behavior, University of California, Irvine, USA.
Journal of molecular evolution
|January 8, 2024
概括
长非编码RNAs (lncRNAs) MALAT1和NEAT1是高度表达的瘤基因. 我们的研究表明,NEAT1在四足动物中广泛存在,而MALAT1在脊椎动物中发现,纠正了关于它们进化存在的先前假设.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 长非编码RNAs (lncRNAs) 调节基因表达和细胞过程.
- 马拉特1和NEAT1高度表达,无处不在的瘤性 lncRNA 参与转录和拼接.
- 之前的研究表明,NEAT1 (哺乳动物) 和MALAT1 (除鸟类外的gnathostomes) 的进化存在有限.
研究的目的:
- 重新评估MALAT1和NEAT1 lncRNAs的进化分布.
- 为了澄清MALAT1/NEAT1基因组位置的起源和进化史.
- 通过进化时间来研究 lncRNA 功能的变化和连续性.
主要方法:
- 对 lncRNA 序列的遗传学分析.
- 基因组和RNA序列数据的结构意识注释.
- 跨多种脊椎动物物种的比较基因组学.
主要成果:
- NEAT1存在于四足动物中,不包括鸟类和类动物,在这些群体中有独立的损失.
- MALAT1在包括鸟类在内的所有主要 gnathostome 类中都被确定.
- 重建了MALAT1/NEAT1位点从软骨鱼到哺乳动物的进化史.
结论:
- NEAT1和MALAT1的进化存在比以前理解的更广泛.
- 在鸟类和毛动物中发生了独立的NEAT1丧失.
- 这项研究提供了对脊椎动物lncRNA演变和功能的见解.
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