关联糖运输体表达和活动以确定孤儿糖基质的运输体
Elisabeth Tamayo1, Basant Nada2,3, Isabell Hafermann2
1Fungal Biotechnology in Wood Science, Holzforschung München, TUM School of Life Sciences, Technical University of Munich, Freising, Germany. tamayo@hfm.tum.de.
Applied microbiology and biotechnology
|January 8, 2024
概括
丝状真菌可以代谢废糖,但许多糖载体仍然未知. 这项研究通过将基因表达与糖摄取相关联,确定了Neurospora crassa中的新的糖载体,帮助废物利用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 丝状真菌具有很大的潜力,可以在农业和食品副产品中代谢废糖.
- 然而,这些真菌中大量的假定糖运输体,包括Neurospora crassa中的30个,仍然没有表征.
- 了解这些载体对于优化废物流的价值化和开发生物技术应用至关重要.
研究的目的:
- 通过将输送物基因表达与糖吸收概况相关联,在Neurospora crassa中识别新型糖载体.
- 阐明特定糖的运输系统,包括乳制品行业副产品乳糖.
- 为了提供一个框架"deorphanizing"糖输送器和识别非特征输送器的基板.
主要方法:
- 在N. crassa.的各种诱导条件下,将44个预测的糖载体基因的表达特征与糖吸收率相关联.
- 对真菌糖载体进行全面的遗传学分析.
- 分析特定糖的运输者候选物,如银酸,阿拉比诺斯和乳糖.
主要成果:
- 在糖的吸收率和运输基因表达之间观察到强烈的相关性,用于糖的专用运输体,如银酸和氨酸.
- 阐明了乳糖的吸收系统,确定了细胞德克斯林载体CDT-1和CDT-2,NCU00809.9的作用很小.
- 还确定了一种参与甘油运输的非MFS运输器,扩大了已知的运输器目录.
结论:
- 关联表达和吸收数据的组合方法,以及植物遗传学分析,对于识别和描述糖载体是有效的.
- 这一策略成功地确定了乳糖和甘油的关键载体,证明了其在发现孤儿糖基质载体方面的实用性.
- 通过了解和利用它们的糖运输系统,可以优化纤维状真菌对食品工业副产品和农业废物的再利用.
相关概念视频
01:27Glucose Transporters
22.8K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.8K
01:55Secondary Active Transport
120.1K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
120.1K
01:30Membrane Proteins
19.6K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
19.6K
01:44The Significance of Membrane Transport
26.1K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
26.1K
01:31Membrane Transporters
11.1K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
11.1K
01:26Glucose Absorption Into the Small Intestine
31.7K
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...
31.7K