揭示了影响Xanthomonas细胞代谢状态的关键步骤,这些细胞经历了编程细胞死亡
Jyoti Tripathi1,2, Satyendra Gautam3,4
1Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, Maharashtra, 400085, India.
概括
在Xanthomonas axonopodis pv.中被编程细胞死亡. 由于不合适的营养条件,甘氨酸是由代谢压力引发的. 确定了涉及分支链氨基酸降解和能量代谢的关键蛋白质,揭示了细胞损伤和DNA修复激活.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 植物病理学 植物病理学
背景情况:
- 编程细胞死亡 (PCD) 在Xanthomonas axonopodis pv. 甘氨酸 (Xag) 以前与代谢压力有关.
- 在Xag中调节这种代谢压力的特定蛋白质仍然未被确定.
研究的目的:
- 研究参与Xag.中PCD代谢应激诱导的关键蛋白质和途径.
- 在营养有限的条件下阐明 Xag 细胞死亡的分子机制.
主要方法:
- 转录组和蛋白组分析被用来全面分析经过PCD的Xag细胞.
- 在PCD诱导媒介中检查了不同的基因和蛋白质表达模式.
主要成果:
- 代谢途径,包括分支链氨基酸降解 (例如,乙-CoA脱酶) 和能量产生的复合物 (ubiquinol:cytochrome c oxidoreductase),在Xag PCD中起着显著的作用.
- 虽然氧化应激反应基因在转录水平上升调节,但蛋白质水平被耗尽,表明过度的细胞损伤.
- 应激反应和DNA修复蛋白被激活,这表明Xag细胞的细胞损伤很大.
结论:
- 在Xag中,编程细胞死亡是由由营养条件对这种植物病原体不利而产生的代谢压力的结果.
- Xag PCD与氨基酸和能量代谢中断有关,加上激活的压力和DNA修复机制.
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