转录因子WOX11和LBD16与基因组脱甲基酶JMJ706一起作用,控制大米的冠根发育
Leping Geng1, Mingfang Tan1, Qiyu Deng1
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
The Plant cell
|January 8, 2024
概括
研究人员确定LBD16是大米冠根形成的关键调节剂. WOX11增强了LBD16,然后形成一个反循环来控制根部发育,改善作物特征.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 冠根对于谷物作物的营养吸收,耐压力和产量至关重要.
- 与WUSCHEL相关的homeobox (WOX) 和侧面器官边界域 (LBD) 转录因子是已知的大米 (Oryza sativa) 冠根发育的调节者.
- 这些因素在皇冠根发育中的确切的功能相互作用尚未完全理解.
研究的目的:
- 确定大米冠根发育的新型调节剂.
- 阐明LBD16在皇冠根形成中的调节机制.
- 了解WOX11,LBD16和JMJ706在调节冠根发育中的相互作用.
主要方法:
- 基因表达分析以确定LBD16作为调节剂.
- 促进体结合试验证实WOX11与LBD16的相互作用.
- 共同免疫沉用于分析蛋白质与蛋白质相互作用 (WOX11,LBD16,JMJ706).
- 在LBD16位点进行质子修饰分析 (H3K9me2).
主要成果:
- 鉴定出LBD16是大米冠根发育的新型调节剂.
- LBD16是WOX11的直接下游目标,从而增强其转录.
- WOX11招募JMJ706去去甲基化LBD16位点,促进其表达.
- LBD16与WOX11相互作用,破坏JMJ706-WOX11复合体并抑制自身的转录,形成一个反循环.
结论:
- 涉及WOX11,LBD16和JMJ706的反监管系统控制了大米冠根的发展.
- LBD16作为一个分子修复剂,微调基因表达,以获得最佳的冠状根形成.
- 了解这种机制可以帮助通过基因操纵改善谷物作物的特征.
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