循环miR-199a和长非编码RNAANRIL作为炎症性肠病的有前途的诊断生物标志物
Randa Erfan1, Olfat G Shaker1, Mahmoud A F Khalil2
1Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Inflammatory bowel diseases
|January 8, 2024
概括
血液中的miR-199a和长非编码RNAANRIL水平升高可能有助于诊断成人炎症性肠病 (IBD),包括克罗恩病 (CD) 和性结肠炎 (UC).
科学领域:
- 生物化学 生物化学
- 遗传学 遗传学 是一个
- 免疫学 免疫学 免疫学
背景情况:
- 炎症性肠病 (IBD),包括克罗恩病 (CD) 和性结肠炎 (UC),是一种慢性免疫媒介疾病.
- 遗传易感性和对肠道细菌的不受控制的炎症反应有助于IBD的发病.
- 血清/血中的microRNAs (miRNAs) 可以反映来自远程器官的表达,在IBD患者中观察到更高的miR-199a水平.
- 长非编码RNA (lncRNA) ANRIL是一种通过核因子 κB 信号传递参与IBD的促炎基因.
研究的目的:
- 研究miR-199a和lncRNAANRIL在IBD.成年患者中的潜在诊断作用.
- 评估miR-199a和ANRIL表达与IBD发生和进展的关联.
主要方法:
- 一项前性队列研究包括67名IBD患者 (35名UC,32名CD) 和30名对照.
- 收集了人口,临床,实验室和治疗数据.
- 在周围血液中测量了瘤亡因子α,miR-199a和ANRIL的水平.
主要成果:
- 在IBD患者的外周血液中,miR-199a和ANRIL都得到了实质性的表达.
- 接收器运行特征 (ROC) 曲线分析显示了两个标记物的预测值.
- miR-199a和ANRIL在诊断UC和CD时表现出不同的敏感性和特异性.
结论:
- 在成年埃及IBD患者 (UC和CD) 的血清中,miR-199a和ANRIL是丰富的.
- 这些分子显示出作为IBD早期诊断的非侵入性生物标志物的潜力.
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