在Primula forbesii Franch中,pfPIN5通过调节细胞长度来促进风格延长
Ying Liu1, Weijia Si1, Sitong Fu1
1State Key Laboratory of Efficient Production of Forest Resources; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture; College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P. R. China.
Annals of botany
|January 8, 2024
概括
在Primula forbesii中,风格的长度是由PfPIN5调节的,PfPIN5是一种auxin流量输送器. 沉默PfPIN5缩短了风格,而其增加的表达延长了它们,揭示了风格发展中的新auxin路径.
科学领域:
- 植物生物学 植物生物学
- 遗传学 是一个遗传学.
- 分子机制的分子机制
背景情况:
- 异构型植物表现出风格二态,针和形在风格和长度上有所不同.
- 之前的研究已经确定了Primula中CYP734A50和brassinosteroids在调节风格长度方面.
- 影响Primula风格长度的因素的全部范围仍然不完全理解.
研究的目的:
- 确定参与调节Primula forbesii的风格长度的新型基因.
- 调查不同表达基因在风格二态化中的作用.
- 阐明底层风格延长的分子机制.
主要方法:
- 使用转录组数据对Primula forbesii中差异表达基因的查.
- 病毒诱导的基因沉默 (VIGS) 来评估基因功能.
- 观察基因沉默后的风格长度和解剖变化.
主要成果:
- 一种辅酶外流输送基因pfPIN5在pin morph风格中高度表达.
- 在型和长型同型植物中抑制PfPIN5导致由于细胞长度减少而导致缩短的风格.
- 在thrum morph植物中抑制CYP734A50的作用提高了PfPIN5的表达和增加了样式长度.
结论:
- 在P. forbesii. 中,pfPIN5在调节风格延长方面发挥着重要作用.
- 奥素途径参与了P. forbesii风格的形成.
- 这项研究为了解风格延长机制提供了一条新的分子途径.
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