番茄Rho植物的零突变体表现出更高的用水效率,而不会影响产量
Mallikarjuna R Puli1, Purity Muchoki1, Adi Yaaran1
1School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv 6997801, Israel.
概括
在护卫细胞中的植物蛋白9 (SlROP9) 控制水损失. SlROP9突变减少了透气,提高了作物用水效率,而不会影响产量.
科学领域:
- 植物生物学 植物生物学
- 农作物科学 农作物科学
- 分子生物学分子生物学
背景情况:
- 提高作物用水效率 (WUE) 对农业至关重要,尤其是在干旱条件下.
- 胃孔调节水的透气和二氧化碳的吸收之间的平衡.
- 植物Rho (ROP) 蛋白质参与植物生长和应激反应.
研究的目的:
- 为了研究番茄植物蛋白9 (SlROP9) 的Rho在护卫细胞中的功能.
- 确定SlROP9对番茄植物口腔调节,透气和用水效率的影响.
主要方法:
- 在番茄中生成和分析SlROP9无基因突变体 (rop9).
- 测量口腔导电性,二氧化碳同化和整个植物的透气.
- 对反应性氧物种 (ROS) 生产和酸 (ABA) 信号通路的研究.
- 在干旱压力下对ROP9表达的分析以及与RBOHB的相互作用.
- 实地实验以评估 rop9 突变物中的用水效率和果产.
主要成果:
- SlROP9无突变体表现出减少的口腔导电性和整个植物的透气性,特别是在高水不足的情况下.
- 光合作用二氧化碳同化在rope9突变体中基本上没有受到影响.
- 在 rop9 突变体中,增加的口腔关闭与增加的 ROS 生产有关,这取决于 RBOH 活动,独立于 ABA.
- 干旱压力抑制了番茄中的ROP9表达,而ABA则抑制了Arabidopsis守护细胞中的ROP信号传递.
- 实地试验表明,rop9突变物提高了水利用效率,而不会影响水果产量.
结论:
- 在番茄护卫细胞中,SlROP9在调节口腔关闭和透气方面发挥着至关重要的作用.
- SlROP9-RBOH通路是口腔对缺水反应的关键调节者,独立于ABA.
- SlROP9是一种有希望的基因标,可以提高农作物用水效率,从而带来更有弹性的农业.
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