从加拿大农业地表水中分离的Arcobacter butzleri的致病性评估
Izhar U H Khan1, Wen Chen2, Michel Cloutier2
1Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, ON, K1A 0C6, Canada. Izhar.Khan@agr.gc.ca.
BMC microbiology
|January 8, 2024
概括
农业排水污染了地表水的Arcobacter butzleri,对人类和动物构成风险. 大多数菌株表现出抗菌素耐药性 (AMR) 和携带毒性基因,突出了改善农场管理的必要性.
科学领域:
- 环境微生物学环境微生物学
- 兽医公共卫生 兽医公共卫生
- 食品安全 食品安全
背景情况:
- 表面水作为Arcobacter物种的传播途径,影响人类和动物的健康.
- 这项研究调查了Arcobacter butzleri菌株在农业流域地表水中的流行,分布和病原性.
研究的目的:
- 评估Arcobacter butzleri在农业表面水中的流行,分布和病原性.
- 确定抗菌素耐药性 (AMR) 和在孤立菌株中识别病毒性相关基因 (VAG).
- 使用ERIC-PCR分析菌株的遗传相关性及其与AMR和VAGs的关联.
主要方法:
- 从加拿大安大略省东部的11个农业地点分离和鉴定了913种Arcobacter butzleri菌株.
- 抗微生物耐药性分析和检测八个毒性相关基因 (VAG).
- 肠杆菌重复性基因间共识聚合酶链反应 (ERIC-PCR) 用于遗传指纹.
主要成果:
- 所有分离物体对至少一种抗微生物药物表现出耐药性,其中克林达米辛 (99%) 和氨基醇 (77%) 的高率.
- 对四环环素 (1%), gentamycin (2%),西普罗夫洛克萨 (4%) 和红色素 (5%) 的敏感性很高.
- VAGs ciaB,mviN,tlyA和pldA非常常见 (>85%),AMR和特定VAG之间存在显著的关联. 埃里克-PCR发现与AMR和VAG资料相关的遗传相似性.
结论:
- 农业活动是表面水中Arcobacter butzleri污染的可能来源.
- 农场管理实践对于控制Arcobacter butzleri的传播和减轻相关的健康风险至关重要.
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