一种基于MIRA-CRISPR/Cas13a-LFD的新型检测方法,针对Trichomonas vaginalis的重复DNA序列
Zhenke Yang1, Jinghui Wang1, Yiming Qi1
1Xinxiang Key Laboratory of Pathogenic Biology, Department of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.
Parasites & vectors
|January 8, 2024
概括
一个新的MIRA-CRISPR/Cas13a-LFD测试可以准确检测阴道菌 (Trichomonas vaginalis),这是非病毒性传播感染的主要原因. 与现有技术相比,这种快速方便的方法可以改善阴道炎的诊断.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 在全球范围内,Trichomonas vaginalis是最常见的非病毒性传播感染.
- 它与盆腔炎症疾病,不良妊娠结果和艾滋病毒风险增加有关.
- 目前的检测方法昂贵而复杂.
研究的目的:
- 开发一种新的,快速和准确的诊断工具来检测T. vaginalis.
- 与现有方法相比,评估新试验的性能.
主要方法:
- 开发了一种多酶同热速放大-集群定期间隔的短时间的平行边形重复 (MIRA-CRISPR) /Cas13a侧流装置 (LFD) 测定.
- 该测试针对T. vaginalis的特定DNA序列,并在37°C下运行大约1小时.
- 在临床样本上进行了测试,并与显微镜,PCR和培养进行了比较.
主要成果:
- 在MIRA-CRISPR/Cas13a-LFD测定表明检测极限为1 × 10−4 ng/μl.
- 该试验显示了100%的灵敏度和与黄金标准培养方法的良好诊断一致 (kappa=1),类似于嵌套PCR.
- 没有观察到与其他常见微生物的DNA交叉反应.
结论:
- MIRA-CRISPR/Cas13a-LFD测定是一种方便,快速,稳定和准确的T. vaginalis的诊断工具.
- 这种方法显示了改善阴道炎诊断和管理的潜力.
- 它代表了与当前诊断技术相比的重大进步.
相关概念视频
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The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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01:23The Antiviral System of Bacteria and Archaea: CRISPR
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01:18Trichomoniasis
Trichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, one of the most prevalent non-viral sexually transmitted infections in the United States. This extracellular parasite primarily colonizes the lower genitourinary tract in women—particularly the vagina—and in men, the urethra and prostate. Its structural and functional adaptations enable its survival, motility, and pathogenicity within the host environment.Structural Features and Host EntryT.