细胞染色体c水平影响TOR通路,以调节在能量缺乏条件下生长和新陈代谢
María Victoria Canal1, Natanael Mansilla1, Diana E Gras1
1Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina.
The New phytologist
|January 8, 2024
概括
植物中的线粒体功能障碍会触发饥饿信号,减少生长. 恢复拉巴胺素 (TOR) 途径的目标可以挽救生长,即使线粒体能量生产受损,这表明一个关键的沟通环节.
科学领域:
- 植物生物学 植物生物学
- 线粒体生理学线粒体生理学
- 蜂信号传输是如何进行的
背景情况:
- 线粒体功能对于植物生长至关重要,但其与生长调节的联系尚不清楚.
- 了解线粒体如何信号能量状态对于植物发育至关重要.
研究的目的:
- 研究 mitochondrial 状态是如何沟通协调植物代谢和生长的.
- 阐明细胞染色体c (CYTc) 在这个信号通路中的作用.
主要方法:
- 研究了具有减少CYTC-1基因表达 (CYTc缺乏) 的阿拉比多普西斯植物.
- 分析了线粒体膜潜力,ATP含量,氨基酸水平和自.
- 研究了对拉巴胺素 (TOR) 途径的标影响,包括S6激酶 (S6K) 和RPS6A酸化.
- 研究了TOR通路操纵 (过度表达) 对CYTc缺陷突变者的影响.
主要成果:
- 尽管有可用的碳来源,但CYTc缺乏导致线粒体膜潜力和ATP水平降低.
- 植物表现出增加的自由氨基酸,诱导了自,并增强了对营养压力的抵抗力.
- 由于CYTc缺乏,TOR通路激活受损,S6K和RPS6A酸化降低,S6K蛋白水平降低.
- 在缺乏CYTc的植物中,TOR的过度表达挽救了生长和其他受影响的参数,不论线粒体功能如何.
结论:
- 缺少CYTc激活了饥饿反应,并减少了TOR通路的信号传递.
- 工厂可以减少TOR激活作为预防措施,以调整预期能源耗尽的增长.
- 这项研究揭示了一种转导线粒体活动变化到细胞代谢和生长调节的机制.
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