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基于表面增强电化学发光的高灵敏检测马六合绿色

Daifang Wang¹, Ligong Shen², Wenjun Liu²

¹Fujian Vocational College of Bioengineering, Fuzhou, Fujian Province, 350002, China. 408951216@qq.com.

Journal of fluorescence

|January 9, 2024

概括

一个新的传感器使用表面增强的电化学发光 (SEECL) 来检测马拉绿 (MG). 这种高度敏感的方法达到1.0nM的检测极限,符合国家海产品安全标准.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
纳米技术纳米技术

背景情况:

马六合绿 (MG) 是一种合成染料,作为兽药使用,引起了食品安全方面的担忧.
现有的MG检测方法可能缺乏灵敏度或需要标签.

研究的目的:

开发一种新型的,未标记的表面增强电化学发光 (SEECL) 传感器,用于敏感的马拉绿色检测.
为了研究发光灭的机制,以提高检测.

主要方法:

在金电极上使用Ru(bpy) 32+合金-SiO2核心外纳米复合材料 (Au@SiO2-Ru(bpy) 32+) 制造SEECL传感器.
利用电化学发光 (ECL) 和局部表面等离子体共振 (LSPR) 的协同效应来增强信号.
研究MG和Ru(bpy) 32+之间的光共振能量转移 (FRET),用于信号灭.

主要成果:

由于LSPR,Au@SiO2-Ru(bpy) 32+纳米复合材料表现出增强的ECL信号.
马六合绿色有效地通过FRET灭了发光.
对于MG检测,SEECL传感器表现出高灵敏度.

结论:

开发的未标记的SEECL传感器提供了一种灵敏可靠的方法来检测马拉绿色.
达到最低检测度为1.0nM,符合中国在海鲜中兽药残留物的国家标准.
这种传感器有可能在食品安全监测中得到实际应用.

关键词:

电化学发光的发光发生.光是一种光效应.黄金纳米颗粒的使用方法局部化的表面等离子体共振.在表面增强的频谱.

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