与菌体基因的相互作用是β-乳酸酶的成本的基础
1School of Natural Sciences, Massey University, Auckland, New Zealand.
mBio
|January 9, 2024
概括
抗生素耐药性基因 (ARG) 的成本因细菌宿主而异. 发现一种菌体基因relA是必要的,并且足以导致大肠杆菌中bla_TEM-116*ARG的适应性成本. 这突出了影响ARG传播的复杂相互作用.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 抗生素耐药性基因 (ARG) 构成了严重的公共卫生威胁.
- ARG的适应性成本可以因细菌宿主而异,影响它们的维护和传播.
- 了解这些成本的遗传基础及其变异性对于预测ARG动态至关重要.
研究的目的:
- 为了确定β-乳酸酶基因的宿主依赖性适应性成本的遗传基础,bla_TEM-116*.
- 调查菌体基因在调解ARG健身成本中的作用.
- 阐明影响ARG维持和在细菌群体中传播的复杂相互作用.
主要方法:
- 确定了不同Escherichia菌株中bla_TEM-116*ARG的宿主依赖性成本的遗传基础.
- 将ARG演变成一个昂贵的宿主,并确定了补偿突变.
- 将菌体基因relA引入中性宿主,以评估其对ARG成本的影响.
主要成果:
- bla_TEM-116* ARG在一个大肠杆菌菌株中产生了显著的成本,但在其他11种菌株中是中性的.
- 菌体基因relA中的突变迅速补偿了ARG在昂贵的宿主中的健身成本.
- 野生型relA的存在足以诱导ARG在以前中立的主体中的适应性成本.
结论:
- 菌体基因relA是必要的,并且足以解释bla_TEM-116*在某些细菌宿主中的适应性成本.
- 这项研究证明了第一个已知的菌体基因影响ARG成本的实例.
- 菌体和等离子体传播的ARG之间的相互作用强调了自然种群中控制ARG动态的选择性压力的复杂性.
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