近红外光探测器用于单氨酸氧化酶A,具有用于手术内导航的大杆移位
Linhao Zhang1, Rong Zhang1, Xinyue Cong1
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
ACS applied bio materials
|January 9, 2024
概括
一个新的近红外光探针,DDM-NH,已经开发出来,用于高度敏感和特定的检测单胺氧化酶A (MAO-A). 这种探测器能够准确地对活细胞和体内MAO-A活性进行成像,解决目前的检测局限性.
科学领域:
- 生物化学 生物化学
- 分子成像学分子成像学
- 化学生物学 化学生物学
背景情况:
- 单胺氧化酶A (MAO-A) 是一个线粒体的黄蛋白,在神经递质代谢中至关重要.
- 现有的近红外光探测器 (NIR-FPs) 缺乏准确的MAO-A检测和成像所需的特异性和灵敏度.
- 想象MAO-A的挑战阻碍了体外和体内细胞研究.
研究的目的:
- 设计和合成一种新的NIR-FP,DDM-NH,用于特定和敏感地检测MAO-A.
- 评估DDM-NH在活生物系统中检测MAO-A的性能.
- 为了证明DDM-NH在体内成像应用中的潜力.
主要方法:
- 合成了NIR-FP DDM-NH,其中包含了二同位素NIR染料前体和氨酸识别部分.
- 描述DDM-NH的光物理性质,包括排放峰值,斯托克斯转移和响应因子.
- 评估DDM-NH的灵敏度,选择性 (与MAO-B相比) 和检测极限.
- 在HeLa细胞,斑马鱼和瘤携带小鼠中对MAO-A活性进行成像的DDM-NH的应用.
主要成果:
- DDM-NH证明了特定的MAO-A检测,NIR发射峰值在770nm和一个大的斯托克斯转移 (180nm).
- 探测器表现出11倍的响应因子,低检测极限为99.7nm,对MAO-A比MAO-B有很高的亲和力.
- DDM-NH成功地可视化了HeLa细胞,斑马鱼和瘤携带小鼠中的MAO-A活性.
结论:
- DDM-NH是一种敏感和特定的NIR-FP用于MAO-A检测.
- 该探测器可以在活生物系统中准确地对MAO-A进行成像,包括细胞和体内模型.
- DDM-NH显示出MAO-A相关的研究和诊断应用的巨大潜力.
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