糖生产从无毒的lignocellulose水解由一个多抗性工程Candida glycerinogenes
Dongqi Jiang1,2,3, Mengying Wang1,2,3, Xiaohong Zhao1,2,3
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|January 9, 2024
概括
这项研究表明,使用工程酵母,从甘包油解液中有效地生产甘. 该过程具有成本效益和环保性,为工业糖合成提供了可持续的替代方案.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 可持续化学 可持续化学
背景情况:
- 甘油是一种多功能平台化学物质,具有广泛的工业应用.
- 使用低成本的红纤维素生物质,如甘包,用于生产甘是非常可取的.
- 甘糖原菌 (Candida glycerinogenes) 是一种酵母菌株,具有很高的甘生产潜力.
研究的目的:
- 从未经处理的甘包水解剂中改造Candida glycerinogenes以提高甘的产量.
- 为了提高工程酵母菌株中西洛斯的利用率.
- 建立一个具有成本效益和环保的工业糖合成方法.
主要方法:
- 对Candida glycerinogenes进行基因改造,以提高草甘利用率.
- 使用工程酵母菌株 (CgS45) 发酵未经排毒的巴加斯水解盐.
- 糖标位和产量的分析.
主要成果:
- 工程化C. glycerinogenes对未经排毒的水解盐中的抑制性化合物表现出耐药性.
- 发酵实现了 40.3 g/L 的甘油位和 40.4% 的产量.
- 从1公斤包中生产93.5克甘油.
结论:
- 这项研究提供了第一份关于直接从红纤维素生产甘油的报告.
- 这种工程酵母菌株有效地利用了未经排毒的包油解液.
- 这为工业甘生产提供了一种新,可持续和环保的途径.
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