双层覆盖的合的缩纤维,具有圆形的双折射,作为结构光的增强介质

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Fiber-reinforced concrete significantly enhances the structural and nonstructural properties of traditional concrete by incorporating fibers like steel, glass, and polymers. These fibers, varying from natural ones such as sisal and cellulose to manufactured ones like polypropylene and Kevlar, are mixed into hydraulic cement with aggregates. Steel fibers, often preferred for their robustness, contribute to improved ductility, toughness, and post-cracking performance. The concrete is classified...