Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.4K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.4K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

11.3K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
11.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intracellular barriers and receptor masking limit success of a <i>Pseudomonas aeruginosa</i> clinical phage family.

bioRxiv : the preprint server for biology·2026
Same author

CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa.

Cell host & microbe·2026
Same author

CBASS limits bacteriophage production while maintaining cell viability in <i>Pseudomonas aeruginosa</i>.

bioRxiv : the preprint server for biology·2026
Same author

Development of ACE2-tropic-betacoronavirus therapeutics for future pandemic preparedness.

Nature communications·2025
Same author

Exceptionally broad HIV-1 neutralization via bispecific antibody-mediated prepositioning.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

A broad antibody with enhanced HIV-1 neutralization via bispecific antibody-mediated prepositioning.

Nature communications·2025

相关实验视频

Updated: Jul 6, 2025

Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

14.3K

让免疫聚焦成为焦点

Sriharshita Musunuri1,2, Payton A B Weidenbacher1,3, Peter S Kim4,5,6

  • 1Stanford ChEM-H, Stanford University, Stanford, CA, 94305, USA.

NPJ vaccines
|January 9, 2024
PubMed
概括
此摘要是机器生成的。

免疫聚焦策略为针对流感和SARS-CoV-2等病毒的通用疫苗创建有针对性的免疫原体. 了解这些方法可以为未来的病毒威胁带来先进的疫苗.

更多相关视频

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

37.0K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.1K

相关实验视频

Last Updated: Jul 6, 2025

Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

14.3K
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

37.0K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.1K

科学领域:

  • 免疫学 免疫学 免疫学
  • 疫苗学 疫苗学 疫苗学
  • 病毒学 病毒学

背景情况:

  • 幽默性免疫反应可以通过免疫聚焦策略重新定向.
  • 开发针对流感,HIV-1和SARS-CoV-2等高度变化的病毒的通用疫苗是一个关键的挑战.
  • 现有的免疫聚焦方法旨在针对特定的表位,以获得更广泛的保护.

研究的目的:

  • 为了说明疫苗设计的五个主要的免疫聚焦策略.
  • 讨论免疫聚焦中的障碍,例如免疫印记.
  • 突出免疫聚焦的潜力,以创建高分辨率疫苗.

主要方法:

  • 审查和说明五个关键的免疫聚焦策略:交叉菌株增强,马赛克显示,蛋白质剖析,表位基因架构和表位基因掩盖.
  • 对现有示例进行分析,以证明这些策略的应用.
  • 讨论包括免疫印记在内的挑战.

主要成果:

  • 阐明了五种不同的免疫聚焦策略.
  • 这些策略克服病毒变异的潜力通过示例来证明.
  • 像免疫印记这样的障碍被确定为关键挑战.

结论:

  • 免疫聚焦方法的发展和应用对于设计有效疫苗至关重要.
  • 可以开发出高分辨率的疫苗,以防止未来的病毒爆发.
  • 免疫聚焦为广泛的病毒保护提供了一个有希望的方法.